rabbit polyclonal anti yap 1  (Cell Signaling Technology Inc)

Journal: Biomolecules

Article Title: Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro

doi: 10.3390/biom11050675

Figure Lengend Snippet: ( A ) Immunofluorescence images of A549 cells stained for YAP-1 (red) cultured on Thermanox™ Coverslips (first row) and on Elastosil ® Film (second row). Nuclei are stained in blue. Merged images are shown on the right. Magnification 63× ( B ) Percentage of nuclear YAP-1 in A549 cells cultured on Thermanox™ Coverslips or Elastosil ® Film (*** p < 0.001, n = 16).

Article Snippet: Subsequently, cells were incubated overnight at 4 °C with different solutions of primary antibodies: (1) rabbit polyclonal anti-YAP-1 (diluted 1:1000, ab81183, Abcam, Cambridge, UK) to evaluate YAP-1 expression, (2) rabbit cleaved caspase-3 (diluted 1:200, product number 9661, Cell Signaling Technologies, Danvers, MA, USA) to study possible apoptosis, (3) rabbit polyclonal to Prosurfactant Protein C (diluted 1:200, ab90716, Abcam, Cambridge, UK) to investigate surfactant protein C expression and (4) rabbit polyclonal phospho-focal adhesion kinase (p-FAK, diluted 1:200, ab81183, Abcam, Cambridge, UK) to stain focal adhesions (FAs).

Techniques: Immunofluorescence, Staining, Cell Culture